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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
17/05/2022 |
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Actualizado : |
02/12/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
PASSOS, J. R. S.; GUERREIRO, D. D.; OTÁVIO, K. S.; SANTOS-NETO, P. C. DOS; SOUZA-NEVES, M.; CUADRO, F.; NUÑEZ-OLIVERA, R.; CRISPO, M.; BEZERRA, M. J. B.; SILVA, R. F.; LIMA, L. F.; FIGUEIREDO, J. R.; BUSTAMANTE-FILHO, I. C.; MENCHACA, A.; MOURA, A. A. |
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Afiliación : |
JOSÉ RENATO S. PASSOS, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; DENISE D. GUERREIRO, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; KAMILA S. OTÁVIO, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; P. C. DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; FEDERICO CUADRO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; RICHARD NUÑEZ-OLIVERA, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; MARTINA CRISPO, Unidad de Biotecnología en Animales de Laboratorio, Institut Pasteur de Montevideo, Montevideo, Uruguay; MARIA JÚLIA B. BEZERRA, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; RENATO F. SILVA, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais - LAMOFOPA - Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Brazil; LARITZA F. LIMA, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais - LAMOFOPA - Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Brazil; JOSÉ RICARDO FIGUEIREDO, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais - LAMOFOPA - Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Brazil; IVAN C. BUSTAMANTE-FILHO, aboratório de Biotecnologia da Reprodução Animal, Programa de Pós-graduação em Biotecnologia, Universidade do Vale do Taquari, Lajeado, Brazil; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; ARLINDO A. MOURA, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil. |
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Título : |
Global proteomic analysis of preimplantational ovine embryos produced in vitro. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Reproduction in Domestic Animals, 2022, Volume 57, Issue 7; pages 784-797. doi: https://doi.org/10.1111/rda.14122 |
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ISSN : |
0936-6768 |
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DOI : |
10.1111/rda.14122 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 15 February 2022; Accepted 1 April 2022. -- Funding text - The experiments presently described were conducted at the facilities of the (Fundacion IRAUy, Montevideo, Uruguay) and at the (UBAL) of the , Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Finnacial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); the Brazilian Research Council?CNPq (grants # 313160/2017‐1 and 438773/2018‐7); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil. Instituto de Reproducción Animal Uruguay Unidad de Biotecnología en Animales de Laboratorio Institut Pasteur de Montevideo. -- Corresponding author: A. Moura, A.; Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; mail:menchaca.alejo@gmail.com |
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Contenido : |
ABSTRACT. -The present study was conducted to characterize the major proteome of preimplantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2–6 mm), matured and fertilized in vitro and cultured until day six. Proteins were ex- tracted separately from three pools of 45 embryos and separately run in SDS-PAGE. Proteins from each pool were individually subjected to in-gel digestion followed by LC-MS/MS. Three ‘raw files’ and protein lists were produced by Pattern Lab software, but only proteins present in all three lists were used for the bioinformatics analyses. There were 2,262 proteins identified in the 6-day-old ovine
embryos, including al- bumin, zona pellucida glycoprotein 2, 3 and 4, peptidyl arginine deiminase 6, actin cytoplasmic 1, gamma-actin 1, pyruvate kinase, heat shock protein 90 and protein disulfide isomerase, among others. Major biological processes linked to the sheep embryo proteome were translation, protein transport and protein stabilization, and molecular functions, defined as ATP binding, oxygen carrier activity and oxygen bind- ing. There were 42 enriched functional clusters
according to the 2,147 genes (UniProt database). Ten selected clusters with potential association with embryo development included translation, structural constituent of ribosomes, ribosomes, nucleosomes, structural constituent of the cytoskeleton, microtubule-based process, translation initiation factor activity, regulation of translational initiation, cell body and nucleotide biosynthetic process. The most representative KEEG pathways were ribosome, oxida- tive phosphorylation, glutathione metabolism, gap junction, mineral absorption, DNA replication and cGMP-PKG signalling pathway. Analyses of functional clusters clearly showed differences associated
with the proteome of preimplantation (D6) sheep em- bryos generated after in vitro fertilization in comparison with in vivo counterparts (Sanchez et al., 2021; https://doi.org/10.1111/rda.13897), confirming that the quality of in vitro derived blastocysts are unlike those produced in vivo. The present study portrays the first comprehensive overview of the proteome of preimplantational ovine embryos grown in vitro.
© 2022 Wiley-VCH GmbH. MenosABSTRACT. -The present study was conducted to characterize the major proteome of preimplantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2–6 mm), matured and fertilized in vitro and cultured until day six. Proteins were ex- tracted separately from three pools of 45 embryos and separately run in SDS-PAGE. Proteins from each pool were individually subjected to in-gel digestion followed by LC-MS/MS. Three ‘raw files’ and protein lists were produced by Pattern Lab software, but only proteins present in all three lists were used for the bioinformatics analyses. There were 2,262 proteins identified in the 6-day-old ovine
embryos, including al- bumin, zona pellucida glycoprotein 2, 3 and 4, peptidyl arginine deiminase 6, actin cytoplasmic 1, gamma-actin 1, pyruvate kinase, heat shock protein 90 and protein disulfide isomerase, among others. Major biological processes linked to the sheep embryo proteome were translation, protein transport and protein stabilization, and molecular functions, defined as ATP binding, oxygen carrier activity and oxygen bind- ing. There were 42 enriched functional clusters
according to the 2,147 genes (UniProt database). Ten selected clusters with potential association with embryo development included translation, structural constituent of ribosomes, ribosomes, nucleosomes, structural constituent of the cytoskeleton, microtubule-based process, translation initiation factor activity, regulation of translational i... Presentar Todo |
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Palabras claves : |
Embryo development; In vitro fertilization; Mass spectrometry; Oocyte; Ovine; PLATAFORMA SALUD ANIMAL; Proteins. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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Marc : |
LEADER 04571naa a2200409 a 4500
001 1063149
005 2022-12-02
008 2022 bl uuuu u00u1 u #d
022 $a0936-6768
024 7 $a10.1111/rda.14122$2DOI
100 1 $aPASSOS, J. R. S.
245 $aGlobal proteomic analysis of preimplantational ovine embryos produced in vitro.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 15 February 2022; Accepted 1 April 2022. -- Funding text - The experiments presently described were conducted at the facilities of the (Fundacion IRAUy, Montevideo, Uruguay) and at the (UBAL) of the , Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Finnacial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); the Brazilian Research Council?CNPq (grants # 313160/2017‐1 and 438773/2018‐7); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil. Instituto de Reproducción Animal Uruguay Unidad de Biotecnología en Animales de Laboratorio Institut Pasteur de Montevideo. -- Corresponding author: A. Moura, A.; Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; mail:menchaca.alejo@gmail.com
520 $aABSTRACT. -The present study was conducted to characterize the major proteome of preimplantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2–6 mm), matured and fertilized in vitro and cultured until day six. Proteins were ex- tracted separately from three pools of 45 embryos and separately run in SDS-PAGE. Proteins from each pool were individually subjected to in-gel digestion followed by LC-MS/MS. Three ‘raw files’ and protein lists were produced by Pattern Lab software, but only proteins present in all three lists were used for the bioinformatics analyses. There were 2,262 proteins identified in the 6-day-old ovine embryos, including al- bumin, zona pellucida glycoprotein 2, 3 and 4, peptidyl arginine deiminase 6, actin cytoplasmic 1, gamma-actin 1, pyruvate kinase, heat shock protein 90 and protein disulfide isomerase, among others. Major biological processes linked to the sheep embryo proteome were translation, protein transport and protein stabilization, and molecular functions, defined as ATP binding, oxygen carrier activity and oxygen bind- ing. There were 42 enriched functional clusters according to the 2,147 genes (UniProt database). Ten selected clusters with potential association with embryo development included translation, structural constituent of ribosomes, ribosomes, nucleosomes, structural constituent of the cytoskeleton, microtubule-based process, translation initiation factor activity, regulation of translational initiation, cell body and nucleotide biosynthetic process. The most representative KEEG pathways were ribosome, oxida- tive phosphorylation, glutathione metabolism, gap junction, mineral absorption, DNA replication and cGMP-PKG signalling pathway. Analyses of functional clusters clearly showed differences associated with the proteome of preimplantation (D6) sheep em- bryos generated after in vitro fertilization in comparison with in vivo counterparts (Sanchez et al., 2021; https://doi.org/10.1111/rda.13897), confirming that the quality of in vitro derived blastocysts are unlike those produced in vivo. The present study portrays the first comprehensive overview of the proteome of preimplantational ovine embryos grown in vitro. © 2022 Wiley-VCH GmbH.
653 $aEmbryo development
653 $aIn vitro fertilization
653 $aMass spectrometry
653 $aOocyte
653 $aOvine
653 $aPLATAFORMA SALUD ANIMAL
653 $aProteins
700 1 $aGUERREIRO, D. D.
700 1 $aOTÁVIO, K. S.
700 1 $aSANTOS-NETO, P. C. DOS
700 1 $aSOUZA-NEVES, M.
700 1 $aCUADRO, F.
700 1 $aNUÑEZ-OLIVERA, R.
700 1 $aCRISPO, M.
700 1 $aBEZERRA, M. J. B.
700 1 $aSILVA, R. F.
700 1 $aLIMA, L. F.
700 1 $aFIGUEIREDO, J. R.
700 1 $aBUSTAMANTE-FILHO, I. C.
700 1 $aMENCHACA, A.
700 1 $aMOURA, A. A.
773 $tReproduction in Domestic Animals, 2022, Volume 57, Issue 7; pages 784-797. doi: https://doi.org/10.1111/rda.14122
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
22/05/2023 |
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Actualizado : |
24/05/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
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Circulación / Nivel : |
Nacional - -- |
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Autor : |
IRISARRI, P.; PEREYRA, V.; FERNÁNDEZ, A.; TERRA, J.A.; TARLERA, S. |
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Afiliación : |
PILAR IRISARRI, Universidad de la República, Facultad de Agronomía, Departamento de Biología Vegetal, Montevideo, Uruguay; VIRGINIA PEREYRA, Universidad de la República, Facultad de Química, Laboratorio de Ecología Microbiana y Ambiental, Montevideo, Uruguay; ANA FERNÁNDEZ, Universidad de la República, Facultad de Química, Laboratorio de Ecología Microbiana y Ambiental, Montevideo, Uruguay; JOSÉ ALFREDO TERRA FERNÁNDEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVANA TARLERA, Universidad de la República, Facultad de Química, Laboratorio de Ecología Microbiana y Ambiental, Montevideo, Uruguay. |
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Título : |
CH4 and N2O Emissions in a Rice Field: First Measurements in the Uruguayan Productive System. [Emisiones de CH4 y N2O en un arrozal: primeras medidas en el sistema productivo uruguayo]. [Special Issue 25 Years Agrociencia]. |
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Complemento del título : |
Plant Biology. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Agrociencia Uruguay, 2022, vol. 26, NE2, e1083. doi: https://doi.org/10.31285/AGRO.26.1083 -- OPEN ACCESS. |
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ISSN : |
2730-5066 |
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DOI : |
10.31285/AGRO.26.1083 |
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Idioma : |
Inglés |
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Notas : |
Article history: Article originally published in: Agrociencia (Uruguay). 2012;16(2):1-10. doi: https://doi.org/10.31285/AGRO.17.533 -- Correspondence: Silvana Tarlera,
starlera@fq.edu.uy -- Special Issue 25 Years Agrociencia. -- License: This work is licensed under a Creative Commons Attribution 4.0 International License. (https://creativecommons.org/licenses/by/4.0/ ) |
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Contenido : |
ABSTRACT.- Irrigated rice fields are major sources of two important greenhouse gases (GHG), methane and nitrous oxide. As an initial step towards obtaining local information, emissions of CH4 and N2O from rice paddy soil were measured by the static chamber technique in greenhouse and field experiments conducted in eastern Uruguay. In the greenhouse experiment, the effect of two flooding moments (21 and 45 days after emergence) and nitrogen fertilization (0 and 50 kg N ha-1) on gas emissions was studied. Early flooding and nitrogen fertilization tended to increase N2O emissions. In the field experiment, effect of winter soil cover crop and nitrogen fertilization (0 and 82 kg N ha-1) were tested. Higher CH4 fluxes were observed mainly during the reproductive stage of the plant in the N-fertilized treatment with ryegrass winter crop. N2O flux peaked at flushing. Results indicate that the use of cover crops might increase GHG emissions during the rice cycle. Despite differences in agronomic management practices employed in Uruguay, CH4 and N2O fluxes are within magnitudes previ-ously reported for rice fields worldwide. .-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-. RESUMEN.- Los arrozales son fuente de dos importantes gases de efecto invernadero (GEI), metano y óxido nitroso. Como un paso inicial hacia la obtención de información local, se midieron las emisiones de CH4 y N2O del suelo y de las plantas de arroz mediante la técnica de la cámara estática en experimentos en invernáculo y a campo en el este de Uruguay. En el experimento en invernáculo, se estudió el efecto del momento de inundación (21 y 45 días después de la emergencia) y de la fertilización nitrogenada (0 y 50 kg N ha-1) sobre las emisiones. La inundación temprana y la fertilización nitrogenada tendieron a aumentar las emisiones de N2O. En el experimento a campo, se estudió el efecto de la cobertura invernal y de la fertilización nitrogenada (0 y 82 kg N ha-1). Se detectaron mayores flujos de CH4 durante la etapa reproductiva de la planta en el tratamiento fertilizado con cobertura invernal previa de raigrás. El flujo de N2O fue máximo después de los baños. Los resultados indican que el uso del cultivo de cobertura podría incrementar las emisiones de GEI durante el ciclo del arroz. A pesar de las distintas prácticas de manejo del cultivo empleadas en Uruguay, los flujos de CH4 y N2O se encuentran dentro de los valores informados previamente para arrozales de otras partes del mundo. Copyright (c) 2022 Agrociencia Uruguay MenosABSTRACT.- Irrigated rice fields are major sources of two important greenhouse gases (GHG), methane and nitrous oxide. As an initial step towards obtaining local information, emissions of CH4 and N2O from rice paddy soil were measured by the static chamber technique in greenhouse and field experiments conducted in eastern Uruguay. In the greenhouse experiment, the effect of two flooding moments (21 and 45 days after emergence) and nitrogen fertilization (0 and 50 kg N ha-1) on gas emissions was studied. Early flooding and nitrogen fertilization tended to increase N2O emissions. In the field experiment, effect of winter soil cover crop and nitrogen fertilization (0 and 82 kg N ha-1) were tested. Higher CH4 fluxes were observed mainly during the reproductive stage of the plant in the N-fertilized treatment with ryegrass winter crop. N2O flux peaked at flushing. Results indicate that the use of cover crops might increase GHG emissions during the rice cycle. Despite differences in agronomic management practices employed in Uruguay, CH4 and N2O fluxes are within magnitudes previ-ously reported for rice fields worldwide. .-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-. RESUMEN.- Los arrozales son fuente de dos importantes gases de efecto invernadero (GEI), metano y óxido nitroso. Como un paso inicial hacia la obtención de información local, se midieron las emisiones de CH4 y N2O del suelo y de las plantas de arroz mediante la técnica de la cámara estática en experimentos en invernáculo y a cam... Presentar Todo |
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Palabras claves : |
Fertilización N; Gases de efecto invernadero; Greenhouse gases; N fertilization; Rice paddy soil; Suelo inundado cultivado con arroz. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/17167/1/2730-5066-1083.pdf
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Marc : |
LEADER 03911naa a2200277 a 4500
001 1064155
005 2023-05-24
008 2022 bl uuuu u00u1 u #d
022 $a2730-5066
024 7 $a10.31285/AGRO.26.1083$2DOI
100 1 $aIRISARRI, P.
245 $aCH4 and N2O Emissions in a Rice Field$bFirst Measurements in the Uruguayan Productive System. [Emisiones de CH4 y N2O en un arrozal: primeras medidas en el sistema productivo uruguayo]. [Special Issue 25 Years Agrociencia].$h[electronic resource]
260 $c2022
500 $aArticle history: Article originally published in: Agrociencia (Uruguay). 2012;16(2):1-10. doi: https://doi.org/10.31285/AGRO.17.533 -- Correspondence: Silvana Tarlera, starlera@fq.edu.uy -- Special Issue 25 Years Agrociencia. -- License: This work is licensed under a Creative Commons Attribution 4.0 International License. (https://creativecommons.org/licenses/by/4.0/ )
520 $aABSTRACT.- Irrigated rice fields are major sources of two important greenhouse gases (GHG), methane and nitrous oxide. As an initial step towards obtaining local information, emissions of CH4 and N2O from rice paddy soil were measured by the static chamber technique in greenhouse and field experiments conducted in eastern Uruguay. In the greenhouse experiment, the effect of two flooding moments (21 and 45 days after emergence) and nitrogen fertilization (0 and 50 kg N ha-1) on gas emissions was studied. Early flooding and nitrogen fertilization tended to increase N2O emissions. In the field experiment, effect of winter soil cover crop and nitrogen fertilization (0 and 82 kg N ha-1) were tested. Higher CH4 fluxes were observed mainly during the reproductive stage of the plant in the N-fertilized treatment with ryegrass winter crop. N2O flux peaked at flushing. Results indicate that the use of cover crops might increase GHG emissions during the rice cycle. Despite differences in agronomic management practices employed in Uruguay, CH4 and N2O fluxes are within magnitudes previ-ously reported for rice fields worldwide. .-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-. RESUMEN.- Los arrozales son fuente de dos importantes gases de efecto invernadero (GEI), metano y óxido nitroso. Como un paso inicial hacia la obtención de información local, se midieron las emisiones de CH4 y N2O del suelo y de las plantas de arroz mediante la técnica de la cámara estática en experimentos en invernáculo y a campo en el este de Uruguay. En el experimento en invernáculo, se estudió el efecto del momento de inundación (21 y 45 días después de la emergencia) y de la fertilización nitrogenada (0 y 50 kg N ha-1) sobre las emisiones. La inundación temprana y la fertilización nitrogenada tendieron a aumentar las emisiones de N2O. En el experimento a campo, se estudió el efecto de la cobertura invernal y de la fertilización nitrogenada (0 y 82 kg N ha-1). Se detectaron mayores flujos de CH4 durante la etapa reproductiva de la planta en el tratamiento fertilizado con cobertura invernal previa de raigrás. El flujo de N2O fue máximo después de los baños. Los resultados indican que el uso del cultivo de cobertura podría incrementar las emisiones de GEI durante el ciclo del arroz. A pesar de las distintas prácticas de manejo del cultivo empleadas en Uruguay, los flujos de CH4 y N2O se encuentran dentro de los valores informados previamente para arrozales de otras partes del mundo. Copyright (c) 2022 Agrociencia Uruguay
653 $aFertilización N
653 $aGases de efecto invernadero
653 $aGreenhouse gases
653 $aN fertilization
653 $aRice paddy soil
653 $aSuelo inundado cultivado con arroz
700 1 $aPEREYRA, V.
700 1 $aFERNÁNDEZ, A.
700 1 $aTERRA, J.A.
700 1 $aTARLERA, S.
773 $tAgrociencia Uruguay, 2022, vol. 26, NE2, e1083. doi: https://doi.org/10.31285/AGRO.26.1083 -- OPEN ACCESS.
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